Comprehensive Resources for Cancer NGS Data Analysis

Tools in Cancer Epigenomics

ChIP Seq

  1. MACS : Model-based Analysis of ChIP-Seq (MACS) on short reads sequencers such as Genome Analyzer (Illumina / Solexa).

  2. Peakseq : program for identifying and ranking peak regions in ChIP-Seq experiments.

  3. S-Mart : An intuitive and lightweight tool, performing several tasks that are usually required during the analysis of mapped RNA-Seq and ChIP-Seq reads, including data selection and data visualization.

  4. SICER : Package for identification of enriched domains from histone modification ChIP-Seq data.

  5. MEME-ChIP : Tool designed especially for discovering motifs in LARGE (50MB maximum) sets of short (around 500bp) DNA sequences centered on locations of interest such as those produced by ChIP-seq experiments.

  6. GEM : A Java software for studying protein-DNA interaction using ChIP-seq/ChIP-exo data.

  7. DREME : A tool for motif discovery in transcription factor ChIP-seq data .

Bisulphite Seq

  1. Bis-SNP : Bisulfite Space genotyper and Methylation caller.

  2. Bsmap : A short reads mapping software for bisulfite sequencing reads.

  3. BRAT : An accurate and efficient tool for mapping short bisulfite-treated reads obtained from the Solexa-Illumina Genome Analyzer.

  4. BatMeth : Improved mapper for bisulfite sequencing reads on DNA methylation.

  5. B-SOLANA : A fast and accurate all-in-one approach including alignment and methylation calling of two-base encoding (colorspace) bisulfite sequencing data.

  6. PASS-bis : A bisulfite aligner suitable for whole methylome analysis of Illumina and SOLiD reads.

  7. Bismark : A tool to map bisulfite converted sequence reads and determine cytosine methylation states.

  8. Kismeth : An online tool for analysis of bisulphite treated reads.

  9. BS Seeker : Performs accurate and fast mapping of bisulfite-treated short reads .

Methylation

  1. NGSmethPipe : A tool to generate high-quality methylation maps.

  2. BSmooth-align : A tool for alignment of bisulfite sequence reads and tabulation of read-level methylation measurements.

  3. Methylkit : An R package for DNA methylation analysis and annotation from high-throughput bisulfite sequencing.

  4. Methylumi : This package provides classes for holding and manipulating Illumina methylation data.

  5. MethylCoder : Performs methylation analysis in single base pair resolution.